Objectives To analyze the distribution and drug resistance of pathogens cultured in hospitalized patients of department of urology so as to provide scientific evidence forrational using of antibiotics in clinic.Methods Clincal data of urine culture and drug resistance of pathogens of inpatients in urology department from January 2014 to June 2015 in our hospital were retrospectively analyzed.Results A total of 1624 urine samples were tested,among them,532 pathogens were isolated and the positive rate was 32.76％.566 pathogenic bacteria strains were isolated from 532 positive samples,of which 499 gram-negative bacilli strains(79.33％),93 gram-positive cocci strains (16.43％) and 24 fungal strains (4.24％).Additionally,top 5 isolation rates of bacteria were Escherichia coil (53.53％),Enterococcus faecium (5.65％),Pseudomonas aeruginosa (4.77％),Enterococcus faecalis (4.24％) and Klebsiella pneumoniae (3.89％).Moreover,E.coli was highly resistant to cephalosporins,quinolones,tetracyclines and sulfonamides.E.faecium was highly resistant to ampicillin,gentamicin with high concentration and quinolones.Conclusions The pathogen cultured in hospitalized patients of department of urology is manily E.coli which has high resistance to some drugs.Thus,it is important to take antibiotics appropriately based on monitoring urine culture,analyzing and summarizing drug resistance of bacteria frequently.