Objective:To investigate the effects of miR-139-5 p on the growth,cell colony formation,invasion and migration ability of ovarian cancer SKOV3 cells and its mechanism.Methods:qRT-PCR was used to detect the expressions of miR-139-5 p in ovarian cancer tissues and ovarian cancer cells SKOV3.miR-139-5 p mimic was transfected into SKOV3 cells,cell viability,cell colony formation,invasion and migration were detected by WST colorimetric assay,colony formation assay and Transwell assay,respectively.TargetScan online software was used to screen the potential target NFAT of miR-139-5 p,and further verify.Results:MiR-139-5 p expression were abnormally decreased in human ovarian cancer tissues and SKOV3 cells.MiRNA-139-5 p overexpression significantly reduced the growth,cell colony formation,migration and invasion of SKOV3 cells.NFAT was a target gene for miR-139-5 p.Overexpression of NFAT could reverse the inhibition of miR-139-5 p overexpression on SKOV3 cell colony formation,invasion ability,invasion and migration.Conclusion:MiR-139-5 p inhibits the growth,cell colony formation,invasion and migration of ovarian cancer SKOV3 cells by targeting NFAT.