AimTo investigate the effect of receptor-interacting serine/threonine kinase 1 (RIPK1) on the progression of osteoarthritis (OA) cases. MethodsChondrocytes were divided into blank control group (NC), interleukin-1β group (IL-1β group) and IL-1β+RIPK1 short hairpin RNA adenovirus vector group (IL-1β+sh-RIPK1 group). Primary chondrocytes were stimulated with IL-1β, and the expression of RIPK1 was knocked down by adenovirus. The expression levels of inflammatory markers and aging genes were detected in chondrocytes. In vivo, the OA model was constructed, and the RIPK1 expression in articular cartilage was knocked down by adenovirus. The pathological progression, SA-β-Gal staining and senescence associated genes were observed by western blotting and qRT-PCR. ResultsIn vitro cell experiment, IL-1β stimulation can significantly up-regulate the inflammatory phenotype of chondrocytes, However, after RIPK1 knockdown, the expression levels of inflammatory markers were significantly downregulated, and the aging phenotype of chondrocytes was also significantly down-regulated (P＜0.05). At the same time, the same results were obtained in vivo animal experiments. ConclusionKnocking down RIPK1 delays the pathological progression of OA by inhibiting chondrocyte aging level.