Endotracheal suctioning is a most frequent invasive procedure in neonates undergoing mechanical ventilation. The procedure includes the patient preparation, airway suctioning and follow-up care, which may associated with adverse events. Based on the Grading of Recommendations Assessment, Development and Evaluation (GRADE), as well as the related research both in China and overseas, the clinical practice guidelines of endotracheal suctioning in neonates with mechanical ventilation is developed in order to promote the standard implementation of this operation and ensure patients' safety.