Objective To investigate the clinical effect between the ureteral catheter and conical guide wire in treatment of ureteral middle and upper segment.Methods 150 patients were randomly divided into control group (n = 75) and observation group (n = 75). The operation time, length of hospital, hospitalization costs, migration of ureteral calculi, postoperative hematuria, fever, renal colic and the calculi clearance rates on one month after surgery were compared between the two groups.Results There were significant differences in operation time and hospitalization costs between the two groups. There were no significant differences in length of hospital stay, migration of ureteral calculi, postoperative complications rate and the calculi clearance rates on one month after surgery between the two groups.Conclusion Ureteral catheter and conical guide wire in treatment of ureteral middle and upper segment with definite therapeutic effect and the complication is few. The ureteral catheter has a longer operation time than conical guide wire, but the conical guide wire treatment cost is relatively high.