Objective To investigate the clinical value of serum lipase (LPS) and amylase (AMY) combined with C reactive protein (CRP) test in the diagnosis of acute pancreatitis.Methods A total of 120 patients with acute pancreatitis admitted to Pingdingshan City Hospital of Traditional Chinese Medicine from August 2021 to February 2023 were selected as the observation group, who were divided into mild group (n=70) and severe group (n=50) according to the severity of the pancreatitis, and the healthy subjects within the same time were selected as the control group (n=60). The three groups of subjects were performed the combined test of LPS, AMY and CRP, and the test results were analyzed.Results LPS, AMY and CRP levels in the observation group were significantly higher than those in the control group (P<0.05). LPS, AMY and CRP levels were higher in severe group than mild group (P<0.05). The combined test showed the highest specificity of 96.67%, and the sensitivity of both combined test and CRP was 95.00%.Conclusion The combined detection of LPS, AMY and CRP in the early diagnosis of acute pancreatitis patients has high specificity and sensitivity, which is suitable for clinical use.